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Aim: Antibiotic resistance in Mycobacterium abscessus renders treatment poorly effective. Despite erm(41)-gene-mediated macrolide resistance, treatment with azithromycin or clarithromycin is recommended. It is contested whether macrolides differ in erm(41) induction. We determine whether this is the case. Methods: M. abscessus CIP104536 was used. Minimum inhibitory concentrations of clarithromycin and azithromycin were determined. Time-kill kinetics of M. abscessus exposed to azithromycin or clarithromycin were performed and RNA was isolated at predetermined intervals for erm(41) quantification. Results: Minimum inhibitory concentrations increased >30-fold. Time-kill kinetics showed a temporary bacteriostatic effect, abrogated by induced resistance. Erm(41) expression was increased following exposure to either macrolide for 7 days. Conclusion: Both macrolides induce resistance similarly, and this should not be an argument in choosing either macrolide for therapy.

Original publication

DOI

10.2217/fmb-2018-0310

Type

Journal

Future microbiology

Publication Date

06/2019

Volume

14

Pages

749 - 755

Addresses

Department of Medical Microbiology & Radboudumc Center for Infectious Diseases, Radboud University Medical Center, Gelderland, The Netherlands.

Keywords

Macrolides, Azithromycin, Clarithromycin, RNA, Bacterial, RNA, Messenger, Anti-Bacterial Agents, Microbial Sensitivity Tests, Gene Expression Profiling, Drug Resistance, Bacterial, Transcriptional Activation, Mycobacterium abscessus