Infrared Spectra of the Thymidine 13C2 and 13C4 Isotopes in H2O and D2O.
Peng H., Ferneyhough Z., Hilber S., Naghi AM., Shelor CP., Kreutz C., Wang L., Matousek P., Stelling AL.
Here we report the synthesis of two new 13C thymidine (T) isotopes labeled at two key positions in its structure, C2═O and C4═O, and the first IR spectra of each T isotope in H2O and in D2O. Our experimental isotope shifts and normal mode calculations indicate some structurally sensitive modes may be more localized than previously thought, making them regiosensitive probes of thymidine's chemical environment. These results provide new physical insight into the normal modes of thymidine, and will guide future studies to determine how these structurally and environmentally sensitive probe vibrations are impacted by the duplex environment.