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<ns4:p><ns4:bold>Background:</ns4:bold> Central to the successful elimination of <ns4:italic>Plasmodium falciparum</ns4:italic> malaria, are tests with superior capability of diagnosing low-density parasitaemias. Empirical evidence on the performance of the commonly available diagnostics (light microscopy (LM), rapid diagnostic tests (RDT) and polymerase chain reaction (PCR)) is needed to better inform case management and surveillance activities within primary health care settings where elimination of <ns4:italic>falciparum</ns4:italic> malaria is targeted. The objective of this study was to estimate the sensitivity (Se) and specificity (Sp) and predictive values of LM, RDT and PCR tests for <ns4:italic>P. falciparum</ns4:italic> infection, while evaluating the effect of specific covariates on the accuracy of the tests.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> The study enrolled 1,563 children via a cross-sectional survey for asymptomatic malaria and those presenting with symptomatic malaria to the Ngerenya dispensary, Kilifi County between March and December 2014. A Bayesian latent class model (BLCM) was fitted to the participants’ diagnostic data obtained from blood samples that were screened for the presence of <ns4:italic>P. falciparum</ns4:italic> using the three tests.</ns4:p><ns4:p> <ns4:bold>Results:</ns4:bold> The PCR assay registered a higher Se (97.6% [92.0; 99.7]) than LM (84.0% [74.8; 91.0]) but similar to RDT (92.2% [84.4; 97.0]). However, the assay showed a similar Sp (98.9% [98.2; 99.4]) to both RDT (99.4% [98.9; 99.7]) and LM (99.5% [99.0; 99.8]). Regarding predictive values, the tests yielded statistically similar estimates of Positive and negative predictive values (PPV and NPV). A serial interpretation of the results of RDT and LM raised the PPVs and NPVs to &gt;98%.</ns4:p><ns4:p> <ns4:bold>Conclusions:</ns4:bold> LM and RDT tests afford high Se and Sp in a low<ns4:italic> P. falciparum</ns4:italic> prevalence setting. A serial combination of the tests assures high PPV and NPV estimates. These elements, coupled with the wide deployment and affordability of the tests, lend the tests useful for guiding clinical care and surveillance activities for <ns4:italic>P. falciparum</ns4:italic> within elimination settings.</ns4:p>

Original publication

DOI

10.12688/wellcomeopenres.15204.1

Type

Journal

Wellcome Open Research

Publisher

F1000 ( Faculty of 1000 Ltd)

Publication Date

08/04/2019

Volume

4

Pages

67 - 67