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ABSTRACT The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.

Original publication

DOI

10.1128/jcm.00811-09

Type

Journal

Journal of Clinical Microbiology

Publisher

American Society for Microbiology

Publication Date

12/2009

Volume

47

Pages

4173 - 4175