Simultaneous Quantification of Plasmodium Antigens and Host Factor C-Reactive Protein in Asymptomatic Individuals with Confirmed Malaria by Use of a Novel Multiplex Immunoassay
Jang IK., Tyler A., Lyman C., Kahn M., Kalnoky M., Rek JC., Arinaitwe E., Adrama H., Murphy M., Imwong M., Ling CL., Proux S., Haohankhunnatham W., Rist M., Seilie AM., Hanron A., Daza G., Chang M., Das S., Barney R., Rashid A., Landier J., Boyle DS., Murphy SC., McCarthy JS., Nosten F., Greenhouse B., Domingo GJ.
Malaria rapid diagnostic tests (RDTs) primarily detect Plasmodium falciparum antigen histidine-rich protein 2 (HRP2) and the malaria-conserved antigen lactate dehydrogenase (LDH) for P. vivax and other malaria species. The performance of RDTs and their utility is dependent on circulating antigen concentration distributions in infected individuals in a population in which malaria is endemic and on the limit of detection of the RDT for the antigens.