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We report the molecular identification, cloning and initial biological characterization of 12 full-length HIV-1 subtype A, D and A/D recombinant transmitted/founder (T/F) genomes. T/F genomes contained intact canonical open reading frames and all T/F viruses were replication competent in primary human T-cells, although subtype D virus replication was more efficient (p<0.05). All 12 viruses utilized CCR5 but not CXCR4 as a co-receptor for entry and exhibited a neutralization profile typical of tier 2 primary virus strains, with significant differences observed between subtype A and D viruses with respect to sensitivity to monoclonal antibodies VRC01, PG9 and PG16 and polyclonal subtype C anti-HIV IgG (p<0.05 for each). The present report doubles the number of T/F HIV-1 clones available for pathogenesis and vaccine research and extends their representation to include subtypes A, B, C and D.

Original publication

DOI

10.1016/j.virol.2012.10.009

Type

Journal

Virology

Publication Date

02/2013

Volume

436

Pages

33 - 48

Addresses

Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Keywords

T-Lymphocytes, Cells, Cultured, Humans, HIV-1, HIV Infections, Receptors, CCR5, Antibodies, Monoclonal, HIV Antibodies, Cloning, Molecular, Sequence Alignment, Sequence Analysis, DNA, Virus Replication, Founder Effect, Genome, Viral, Open Reading Frames, Female, Male