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Raman spectroscopy is an optical technique able to interrogate biological tissues, giving us an understanding of the changes in molecular structure that are associated with disease development. The Kerr-gated Raman spectroscopy technique uses a picosecond pulsed laser as well as fast temporal gating of collected Raman scattered light. Prostate samples for this study were obtained by taking a chip at the transurethral resection of the prostate (TURP), and bladder samples from a biopsy taken at transurethral resection of bladder tumor (TURBT) and TURP. Spectra obtained through the bladder and prostate gland tissue, at different time delays after the laser pulse, clearly show change in the spectra as depth profiling occurs, eventually showing signals from the uric acid cell and urea cell, respectively. We show for the first time, using this novel technique, that we are able to obtain spectra from different depths through both the prostate gland and the bladder. This has major implications in the future of Raman spectroscopy as a tool for diagnosis. With the help of Raman spectroscopy and Kerr gating, it may be possible to pick up the spectral differences from a small focus of adenocarcinoma of the prostate gland in an otherwise benign gland, and also stage the bladder cancers by assessing the base of the tumor post resection.

Original publication

DOI

10.1117/1.1991848

Type

Journal

Journal of biomedical optics

Publication Date

07/2005

Volume

10

Addresses

Gloucestershire Royal Hospital, Biophotonics Research Group, Pullman Court, Great Western Road, Gloucester GL 1 3NN, United Kingdom.

Keywords

Humans, Adenocarcinoma, Prostatic Neoplasms, Artifacts, Spectrum Analysis, Raman, Sensitivity and Specificity, Reproducibility of Results, Algorithms, Male, Urinary Bladder Neoplasms, Biomarkers, Tumor