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The nucleotide 5'-dGMP and polynucleotide poly(dGdC).poly(dGdC) have been irradiated by using a 200-fs, 200-nm laser pulses and spectrally characterized by using time-resolved infrared spectroscopy. Under the experimental conditions, 200-nm excitation generates both electronic excited states and radical cations through photoionization; the former decay rapidly to vibrationally hot ground state. By using infrared signatures we have been able to follow these processes, and at time scales of >1 ns we observe an infrared marker band at 1,702 cm(-1) within both 5'-dGMP and the polynucleotide assigned to a photoionized product of guanine. This transient has also been reproduced through indirect chemistry through the reaction with photogenerated carbonate radical with 5'-dGMP. The ability to use time-resolved infrared spectroscopy in this way paves the way for developing solution-phase studies to investigate both direct and indirect radiation chemistry of DNA.

Original publication

DOI

10.1073/pnas.0506860103

Type

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Date

07/02/2006

Volume

103

Pages

2150 - 2153

Addresses

School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, United Kingdom.

Keywords

DNA Damage, DNA, Polydeoxyribonucleotides, Spectroscopy, Fourier Transform Infrared, Lasers