Real-time sampling of travelers shows intestinal colonization by multidrug-resistant bacteria to be a dynamic process with multiple transient acquisitions
Kantele A., Kuenzli E., Dunn SJ., Dance DAB., Newton PN., Davong V., Mero S., Pakkanen SH., Neumayr A., Hatz C., Snaith A., Kallonen T., Corander J., McNally A.
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Antimicrobial resistance (AMR) is highly prevalent in low- and middle-income countries. International travel contributes substantially to the global spread of intestinal multidrug-resistant gram-negative (MDR-GN) bacteria. Of the 100 million annual visitors to tropical countries, 30–70% become colonized by MDR-GN bacteria. The phenomenon has been well documented, but since sampling has only been conducted after travelers’ return home, data on the actual colonization process are scarce.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A group of 20 European volunteers visiting Lao People’s Democratic Republic for three weeks provided daily stool samples and filled in daily questionnaires. Acquisition of extended-spectrum beta-lactamase-producing gram-negative bacteria (ESBL-GN) was examined by selective stool cultures followed by whole-genome sequencing (WGS) of isolates.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>While colonization rates were 70% at the end of the study, daily sampling revealed that all participants had acquired ESBL-GN at some time point during their overseas stay, the status varying day by day. WGS analysis ascribed the transient pattern of colonization to sequential acquisition of new strains, resulting in a loss of detectable colonization by the initial MDR-GN strains. All but one participant acquired multiple strains (2–5). Of the total of 83 unique strains identified (53 <jats:italic>E. coli</jats:italic>, 10 <jats:italic>Klebsiella</jats:italic>, 20 other ESBL-GN species), some were shared by as many as four subjects.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>This is the first study to characterize in real time the dynamics of acquiring MDR-GN during travel. Our data show multiple transient colonization events indicative of constant microbial competition.</jats:p></jats:sec><jats:sec><jats:title>Summary</jats:title><jats:p>While 14 of 20 Europeans carried multidrug-resistant bacteria at the end of their 3-week visit to Laos, whole-genome sequencing of daily stool samples revealed acquisition by all, involving multiple transient acquisitions with a potential for longer MDR-GN colonization.</jats:p></jats:sec>