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The enzyme-linked immunosorbent assay (ELISA) is a reliable and relatively low-cost method for measuring soluble ligands such as antibodies and proteins in biological samples. For analysis of specific antibodies in serum, a capture antigen is immobilized onto a solid polystyrene surface from which it can capture the antibodies. The captured antibodies are subsequently detected using a secondary antibody conjugated to an enzyme. Detection is accomplished by addition of a colorimetric substrate, and the readout is absorbance (optical density). Here, we provide a detailed standardized ELISA protocol for the quantification of antibodies against malaria antigens.

Original publication

DOI

10.1007/978-1-4939-9550-9_6

Type

Journal

Methods in molecular biology (Clifton, N.J.)

Publication Date

01/2019

Volume

2013

Pages

83 - 90

Addresses

KEMRI Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya. LMurungi@kemri-wellcome.org.