Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Dialysate effluent from 41 patients presenting with 54 episodes of CAPD peritonitis was examined by four culture techniques, three employing methods for the lysis of peritoneal leucocytes. The most efficient method employed a distilled water lysis-centrifugation technique (81% of episodes culture-positive). Filtration (without leucocyte lysis) was less effective (74% culture-positive). The results of a mechanical leucocyte lysis technique were less satisfactory still, the culture positive rate of 74% being compromised by the recovery of the infecting organism in low numbers, and by an association with a high incidence of plate contamination. The results of a bile-salt lysis technique were the least satisfactory (67% culture-positive).

Original publication

DOI

10.1016/0195-6701(90)90115-5

Type

Journal

The Journal of hospital infection

Publication Date

10/1990

Volume

16

Pages

263 - 269

Addresses

Department of Medical Microbiology, St Bartholemew's Hospital, London.

Keywords

Leukocytes, Humans, Bacteria, Peritonitis, Water, Bile Acids and Salts, Peritoneal Dialysis, Continuous Ambulatory, Centrifugation, Bacteriological Techniques