Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

To determine whether an ongoing response to Leishmania major would affect the response to a non-cross-reacting, non-leishmanial antigen, susceptible BALB/c mice and resistant C3H mice were infected with L. major parasites expressing Escherichia coli beta-galactosidase (beta-GAL); this parasite was designated L. major-betaGAL. BALB/c and C3H mice responded to infection with L. major-betaGAL by mounting a CD4 T-cell response to both parasite antigens and to the reporter antigen, beta-GAL. The phenotypes of these T cells were characterized after generating T-cell lines from infected mice. As expected, BALB/c mice responded to infection with L. major-betaGAL by producing interleukin 4 in response to the parasite and C3H mice produced gamma interferon (IFN-gamma) in response to the parasite and beta-GAL. Interestingly, however, BALB/c mice produced IFN-gamma in response to beta-GAL. Taken together, these results demonstrate that priming of IFN-gamma-producing cells can occur in BALB/c mice despite the fact the animals are simultaneously mounting a potent Th2 response to L. major.

Original publication

DOI

10.1128/iai.68.2.809-814.2000

Type

Journal

Infection and immunity

Publication Date

02/2000

Volume

68

Pages

809 - 814

Addresses

Department of Tropical Public Health, Harvard School of Public Health, Harvard Medical School, Boston, Massachusetts 02115, USA.

Keywords

CD4-Positive T-Lymphocytes, Th1 Cells, Animals, Mice, Inbred BALB C, Mice, Inbred C3H, Mice, Leishmania major, Leishmaniasis, Cutaneous, beta-Galactosidase, Lipopolysaccharides, Antibodies, Protozoan, Transfection, Macrophage Activation, Interferon-gamma