Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Treatment failures to the first- and second-lines antimalarial drugs chloroquine and sulfadoxine-pyrimethamine have increased in the Purworejo district on the island of Java, Indonesia. A molecular epidemiologic study was conducted to determine the frequency distribution of mutant alleles of the genes associated with the resistance among the isolates of Plasmodium falciparum from the area. Analyses using a polymerase chain reaction and restriction fragment length polymorphism showed that nearly all of the 111 samples carried mutant alleles in genes associated with chloroquine resistance: P. falciparum multi-drug resistance 1 (pfmdrl) 86Y (92%), 1042D (4.5%), and P. falciparum chloroquine resistance transporter (pfcrt) 76T (99.1%). Mutant alleles of the in the dihydrofolate reductase (dhfr) gene were also high (84.7%), either as 108N and 108T or paired with 59R, and 16V, respectively. Mutant alleles in the dihydropteroate synthase gene were the least common, either as a single 437G mutation (35.3%) or paired with 540E (26.5%). These results are consistent with the antimalarial drug resistance situation in the area and emphasize the need for a proper treatment strategy.

Type

Journal

The American journal of tropical medicine and hygiene

Publication Date

12/2003

Volume

69

Pages

614 - 620

Addresses

Eijkman Institute for Molecular Biology, Jakarta, Indonesia. din@eijkman.go.id

Keywords

Animals, Humans, Plasmodium falciparum, Malaria, Falciparum, Tetrahydrofolate Dehydrogenase, Dihydropteroate Synthase, Antimalarials, Polymerase Chain Reaction, Drug Resistance, Multiple, Mutation, Polymorphism, Restriction Fragment Length, Alleles, Indonesia