Shifted Excitation Raman Difference Spectroscopy with Charge-Shifting Charge-Coupled Device (CCD) Lock-In Detection.
Sowoidnich K., Towrie M., Maiwald M., Sumpf B., Matousek P.
Shifted excitation Raman difference spectroscopy (SERDS) can provide effective, chemically specific information on fluorescent samples. However, the restricted ability for fast alternating detection (usually B) ratio two times higher than that achieved with conventional CCD read-out SERDS-SORS. The novel approach overcomes fundamental limitations of conventional CCDs. In conjunction with the inherent capability of the charge-shifting lock-in technique to suppress rapidly varying ambient light interference demonstrated by us earlier it is expected to be particularly beneficial with heterogeneous fluorescent samples in field applications.