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We present the first quantitative study of polymorphic content in a model pharmaceutical formulation using transmission Raman spectroscopy (TRS), and compare the results obtained with those from traditional backscattering geometry. The transmission method is shown to provide a true bulk measurement of the composition, being unaffected by systematic or stochastic sub-sampling issues that can plague traditional backscattering geometries. The accuracy of the quantification of the polymorphs using TRS was shown to surpass considerably that achieved using conventional backscattering mode. For a model-free fit, the TRS method yielded R(2) of 0.996 compared to the backscattering value of 0.802; for a partial least squares fit with a single component the TRS method accounted for 98.09% of the variance in the data and yielded an R(2) of 0.985, compared to 89.65% of the variance and R(2) of 0.804 for the backscattering method.

Original publication

DOI

10.1039/c0an00352b

Type

Journal

The Analyst

Publication Date

09/2010

Volume

135

Pages

2328 - 2333

Addresses

School of Pharmacy, Boots Science Building, University of Nottingham, NG7 2RD, UK.

Keywords

Flufenamic Acid, Anti-Inflammatory Agents, Pharmaceutical Preparations, Crystallization, Spectrum Analysis, Raman, Drug Compounding