Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

<jats:title>ABSTRACT</jats:title><jats:p>Malaria elimination will require interventions that prevent parasite transmission from the human host to the mosquito. Experimentally, this is usually determined by the expensive and laborious<jats:named-content xmlns:xlink="" content-type="genus-species" xlink:type="simple">Plasmodium falciparum</jats:named-content>standard membrane feeding assay (PfSMFA), which has limited utility for high-throughput drug screening. In response, we developed the<jats:named-content xmlns:xlink="" content-type="genus-species" xlink:type="simple">P. falciparum</jats:named-content>dual gamete formation assay (PfDGFA), which faithfully simulates the initial stages of the PfSMFA<jats:italic>in vitro</jats:italic>. It utilizes a dual readout that individually and simultaneously reports on the functional viability of male and female mature stage V gametocytes. To validate, we screen the Medicines for Malaria Venture (MMV) Malaria Box library with the PfDGFA. Unique to this assay, we find compounds that target male gametocytes only and also compounds with reversible and irreversible activity. Most importantly, we show that compound activity in the PfDGFA accurately predicts activity in PfSMFAs, which validates and supports its adoption into the transmission-stage screening pipeline.</jats:p>

Original publication





Antimicrobial Agents and Chemotherapy


American Society for Microbiology

Publication Date





7292 - 7302