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Macrorestriction profile analysis by pulsed-field gel electrophoresis (PFGE) was used to distinguish between seven isolates of Cowdria ruminantium from geographically different areas. Characteristic profiles were generated for each isolate by using the restriction endonucleases KspI, SalI, and SmaI with chromosomal sizes ranging between 1,546 and 1,692 kb. Statistical analysis of the macrorestriction profiles indicated that all the isolates were distinct from each other; these data contribute to a better understanding of the epidemiology of this pathogen and may be exploited for the identification of genotype-specific DNA probes.

Type

Journal

Journal of clinical microbiology

Publication Date

05/2000

Volume

38

Pages

1967 - 1970

Addresses

Onderstepoort Veterinary Institute, Onderstepoort, 0110, South Africa.

Keywords

Cells, Cultured, Animals, Cattle, Ehrlichia ruminantium, Heartwater Disease, DNA Probes, Electrophoresis, Gel, Pulsed-Field, Restriction Mapping, Phylogeny, Genotype, Polymorphism, Restriction Fragment Length, South Africa, Ghana, Senegal, Genetic Variation