Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

<jats:p>Diarrheal disease is a complex syndrome that remains a leading cause of global childhood morbidity and mortality. The diagnosis of enteric pathogens in a timely and precise manner is important for making treatment decisions and informing public health policy, but accurate diagnosis is a major challenge in industrializing countries. Multiplex molecular diagnostic techniques may represent a significant improvement over classical approaches. We evaluated the Luminex xTAG gastrointestinal pathogen panel (GPP) assay for the detection of common enteric bacterial and viral pathogens in Vietnam. Microbiological culture and real-time PCR were used as gold standards. The tests were performed on 479 stool samples collected from people admitted to the hospital for diarrheal disease throughout Vietnam. Sensitivity and specificity were calculated for the xTAG GPP for the seven principal diarrheal etiologies. The sensitivity and specificity for the xTAG GPP were &gt;88% for<jats:named-content content-type="genus-species">Shigella</jats:named-content>spp.,<jats:named-content content-type="genus-species">Campylobacter</jats:named-content>spp., rotavirus, norovirus genotype 1/2 (GI/GII), and adenovirus compared to those of microbiological culture and/or real-time PCR. However, the specificity was low (∼60%) for<jats:named-content content-type="genus-species">Salmonella</jats:named-content>species. Additionally, a number of important pathogens that are not identified in routine hospital procedures in this setting, such as<jats:named-content content-type="genus-species">Cryptosporidium</jats:named-content>spp. and<jats:named-content content-type="genus-species">Clostridium difficile</jats:named-content>, were detected with the GPP. The use of the Luminex xTAG GPP for the detection of enteric pathogens in settings, like Vietnam, would dramatically improve the diagnostic accuracy and capacity of hospital laboratories, allowing for timely and appropriate therapy decisions and a wider understanding of the epidemiology of pathogens associated with severe diarrheal disease in low-resource settings.</jats:p>

Original publication

DOI

10.1128/jcm.03321-15

Type

Journal

Journal of Clinical Microbiology

Publisher

American Society for Microbiology

Publication Date

04/2016

Volume

54

Pages

1094 - 1100