Dr Eunice Nduati
Immune responses to HIV
Africa bears the largest burden of infections, and human response influences the general health of the population, when some people have better disease outcomes. By working within our context, we can identify relevant targets for vaccine development, and also understand the immune response we can expect when implementing an intervention.
I am a research scientist based at the KEMRI Wellcome Trust Research Programme (KWTRP), Kilifi Kenya. I currently hold a Mid-Level-Career fellowship under the Wellcome Trust funded KWTRP/IDEAL Programme and have additional support from the International AIDS Vaccine Initiative (IAVI) to study various aspects of HIV immunology and pathogenesis.
My primary focus is to understand the cellular and molecular mechanisms involved in the generation of antibodies with relevant functions. Specifically, I am interested in understanding B and T follicular helper (Tfh) cell interactions and how these may impact on the quality of the subsequent HIV-specific antibodies produced. The generation of broadly neutralizing antibodies requires many rounds of affinity maturation, an event that occurs in the germinal centre and requires help from Tfh cells, which play a critical role in the formation of germinal centers and providing important signals to B cells. It is therefore reasonable to suggest that the quality of the Tfh cells activated can determine success or failure in the generation of broadly neutralizing antibodies, or antibodies with other protective functions. Understanding the interaction of Tfh cells with B cells therefore remains relevant for the development of antibody-based vaccines. While antibody neutralization breadth takes time to develop, antibodies with other Fc mediated functions appear early in HIV infection and I am also interested in understanding their potential role in early viral control.
As part of our collaborative programme with IAVI, I am interested in establishing immunogenicity testing frameworks for HIV vaccine studies. This involves establishing and coordinating high-risk adult population cohorts and setting up relevant functional assays for both humoral (neutralizing and Fc mediated antibody functional assays) and CD8 mediated function (Viral Inhibition Assays and Interferon gamma ELISPOT). This framework has contributed to HIV prevention and care programming for key populations in Kenya and towards our understanding of host immune responses in the context of natural HIV infection.
Kinetics of naturally induced binding and neutralising anti-SARS-CoV-2 antibody levels and potencies among SARS-CoV-2 infected Kenyans with diverse grades of COVID-19 severity: an observational study
Kimotho J. et al, (2023), Wellcome Open Research, 8, 350 - 350
Safety and immunogenicity of ChAdOx1 nCoV-19 (AZD1222) vaccine in adults in Kenya: a phase 1/2 single-blind, randomised controlled trial
Hamaluba M. et al, (2023), Wellcome Open Research, 8, 182 - 182
Serum immunoglobulin G and mucosal immunoglobulin A antibodies from prepandemic samples collected in Kilifi, Kenya, neutralize SARS-CoV-2 in vitro.
Nyagwange J. et al, (2023), International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 127, 11 - 16
Assessment of a diverse panel of transmitted/founder HIV-1 infectious molecular clones in a luciferase based CD8 T-cell mediated viral inhibition assay.
Fernandez N. et al, (2022), Frontiers in immunology, 13
Coordinated Fc-effector and neutralization functions in HIV-infected children define a window of opportunity for HIV vaccination.
Nduati EW. et al, (2021), AIDS (London, England), 35, 1895 - 1905