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Research Malaria Microscopy Standards (ReMMS) applicable to malaria clinical research studies have been published in Malaria Journal. The paper describes the rationale for proposed standards to prepare, stain and examine blood films for malaria parasites.

Three researchers using microscopes to study malaria parasites © Credit Mehul Dhorda
Microscopists from DRC KIMORU lab reading slides as part of the training for the TRAC2 trial of triple artemisinin-based combination therapies, or TACTs for the treatment of multidrug resistant P. falciparum infections.

The standards complement the methods manual previously published by the World Health Organization and UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR). The standards aim to promote consistency and comparability of data from microscopy performed for malaria research and hence to strengthen evidence for improvements in malaria prevention, diagnostics and treatment.

Microscopy is important in both malaria diagnosis and research. It is used to differentiate between Plasmodium species and stages and to estimate parasite density in the blood – an important determinant of the severity of disease. It is also used to monitor the effectiveness of drugs based on the rate at which parasites recrudesce or are cleared from the blood.

While rapid diagnostic tests have replaced microscopy in some contexts, microscopy remains an essential tool to support clinical diagnosis and research. Other existing guidelines and procedures for clinical microscopy are an excellent resource, but do not adequately address the more stringent requirements for malaria microscopy in research.

In a new paper in Malaria Journal, researchers describe comprehensive standards for undertaking and reporting microscopy in the context of research for malaria parasite detection, identification and quantification. These are complemented by a methods manual and the Obare calculator to aid standardisation in microscopy methods. The standards have been developed by the ReMMS Working Group, a panel of experts convened by TDR and are freely available to the research community.

The standardisation of methods allows direct comparisons from studies conducted across different points in time and location. This facilitates individual participant data meta-analyses, recognised as the gold standard approach to generate evidence for improvements in interventions and hence patient outcomes.

Dr Mehul Dhorda, Head of WWARN’s Asia-Pacific Regional Centre, who led the work says: “Along with the members of the ReMMS Working Group, we extensively reviewed existing methods and standards for malaria microscopy with an aim to generate a coherent set of standards and procedures for research. We hope they will be adopted by research groups, especially in malaria endemic regions where research is most needed, as an aid for quality improvement and harmonisation.”

Read more on the WWARN website

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