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ContextPlant peptides garner attention for their potential antimicrobial properties amid the rising concern over antibiotic-resistant bacteria.ObjectiveThis study investigates the antibacterial potential of crude peptide extracts from 27 Thai plants collected locally.Materials and methodsPeptide extracts from 34 plant parts, derived from 27 Thai plants, were tested for their antimicrobial efficacy against four highly resistant bacterial strains: Streptococcus aureus MRSA, Pseudomonas aeruginosa, Acinetobacter baumannii, and Escherichia coli. The stability of these peptide extracts was examined at different temperatures, and the synergistic effects of two selected plant peptide extracts were investigated. Additionally, the time-kill kinetics of the individual extracts and their combination were determined against the tested pathogens.ResultsPeptides from Allium sativum L. and Allium oschaninii O. Fedtsch (Amaryllidaceae) were particularly potent, inhibiting bacterial growth with MICs ranging from 1.43 to 86.50 µg/mL. The consistent MICs and MBCs of these extracts across various extraction time points highlight their reliability. Stability tests reveal that these peptides maintain their antimicrobial activity at -20 °C for over a month, emphasizing their durability for future exploration and potential applications in addressing antibiotic resistance. Time-kill assays elucidate the time and concentration-dependent nature of these antimicrobial effects, underscoring their potent initial activity and sustained efficacy over time.Discussion and conclusionsThis study highlights the antimicrobial potential of Allium-derived peptides, endorsing them for combating antibiotic resistance and prompting further investigation into their mechanisms.

Original publication

DOI

10.1080/13880209.2024.2395517

Type

Journal

Pharmaceutical biology

Publication Date

12/2024

Volume

62

Pages

666 - 675

Addresses

Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Keywords

Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Allium, Garlic, Peptides, Plant Extracts, Anti-Bacterial Agents, Microbial Sensitivity Tests, Drug Resistance, Bacterial, Drug Synergism, Thailand, Methicillin-Resistant Staphylococcus aureus