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To evaluate a simple and rapid testing strategy to diagnose HIV infection in Ethiopia, we subjected a panel of 688 sera with known HIV serologic status (confirmed by ELISA/WB or double ELISA) to 3 rapid assays: Determine HIV-1/2, Capillus HIV-1/2 and Serocard HIV. Samples were obtained from participants in a cohort study on HIV-infection (72%), from tuberculosis patients (18%) and from participants in surveillance studies among police recruits and commercial sex workers (10%). The panel consisted of 249 HIV-1 positive samples, of which 68 were HIV-1 subtype C and 1 HIV-1 subtype A, and 439 HIV-1 negative samples. Determine and Capillus were 100% sensitive and 99.8% specific, Serocard was 100% sensitive and specific. On retrospective evaluation, both parallel (samples tested simultaneously by two rapid assays) and serial (samples tested by two consecutive rapid assays) testing algorithms were 100% sensitive and specific when compared to ELISA/WB or double ELISA testing strategy. In conclusion rapid assays have high sensitivity and specificity. HIV serodiagnosis based on rapid assays may therefore be a valuable alternative in voluntary counselling and testing centres and in facilities where sophisticated laboratories are not available.

Type

Journal article

Journal

Ethiopian medical journal

Publication Date

04/2002

Volume

40 Suppl 1

Pages

27 - 36

Addresses

Ethio-Netherlands AIDS Research Project (ENARP), P.O.Box 1242, Addis Ababa, Ethiopia. Hailu@enarp.com

Keywords

Humans, HIV-1, Tuberculosis, HIV Infections, Mass Screening, Blotting, Western, Enzyme-Linked Immunosorbent Assay, AIDS Serodiagnosis, Sensitivity and Specificity, Retrospective Studies, Algorithms, Time Factors, Police, Ethiopia, Sex Work